Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Participants’ characteristics at 30 years of age. 1982 Pelotas Birth Cohort, 2012

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Activity Assay

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Mean difference (95% CI) in standardized log adiponectin concentration per unit increase in standardized fat depots for males (A) and females (B). Unadjusted models estimates are represented by grey dots and adjusted models by black squares. Adjusted models included genomic ancestry, smoking status, alcohol intake and other fat depots. SD, standard deviation. Data from the 2012 follow-up of the 1982 Pelotas Birth Cohort.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay, Standard Deviation

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Dose-response relation between fat depots and adiponectin concentration in males. (A) Visceral fat ( P for nonlinear trend = 0.003); (B) deep subcutaneous abdominal fat ( P for nonlinear trend = 0.121); (C) superficial subcutaneous abdominal fat ( P for nonlinear trend = 5*10 −6 ); (D) gluteofemoral fat ( P for nonlinear trend = 3*10 −4 ). SD, standard deviation. Data from the 2012 follow-up of the 1982 Pelotas Birth Cohort.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay, Standard Deviation

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Dose-response relation between fat depots and adiponectin concentration in females. (A) Visceral fat ( P for nonlinear trend = 0.006); (B) deep subcutaneous abdominal fat ( P for nonlinear trend = 0.105); (C) superficial subcutaneous abdominal fat ( P for nonlinear trend = 0.058); (D) gluteofemoral fat ( P for nonlinear trend = 0.037). SD, standard deviation. Data from the 2012 follow-up of the 1982 Pelotas Birth Cohort.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay, Standard Deviation

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Mean difference (95% CI) in log adiponectin concentration per unit increase in fat depots for males (A) and females (B) after multiple imputation

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Causal diagram representing assumed causal relationships in the Mendelian randomization analysis. The solid lines represent the relationships being tested (i.e. effect of WC or HipC on adiponectin concentration using genetic instruments). WC, waist circumference; HipC, hip circumference; SNP, single nucleotide polymorphisms.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Main (A) and sensitivity (B) Mendelian randomization analyses of the mean difference (95% CI) in standardized log adiponectin concentration per unit increase in standardized waist (grey dots) or hip circumference (black squares). Data from GIANT ( n = up to 210 088 individuals) and ADIPOGen ( n = 29 347 individuals) consortia. Adjusted IVW model: IVW method adjusted for hip circumference (in waist circumference model) or waist circumference (in hip circumference model). Waist and hip circumference were adjusted by body mass index before analysis. IVW method, inverse variance method; MR, Mendelian randomization.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay

Journal: International Journal of Epidemiology

Article Title: Obesity-induced hypoadiponectinaemia: the opposite influences of central and peripheral fat compartments

doi: 10.1093/ije/dyx022

Figure Lengend Snippet: Mean difference in standardized log adiponectin concentration according to genetically increased standardized waist (A) or hip (B) circumference. Data from GIANT ( n = up to 210 088 individuals) and ADIPOGen ( n = 29 347 individuals) consortia. Each data point represents betas for SNP-adiponectin (Y axis) and SNP-waist or hip circumference (X axis) association. Predicted values for the main analyses are represented by the full black line (unadjusted IVW method) and full grey line (adjusted IVW method). Predicted values for the sensitivity analyses are represented by the dashed black line (weighted median estimator) and dashed grey line (MR-Egger method). IVW method, inverse-variance weighted method; MR-Egger method, Mendelian randomization-Egger method.

Article Snippet: Adiponectin was assayed with the ELISA Quantikine Human Total Adiponectin Immunoassay kit (R&D Systems, Inc., Minneapolis, USA) and SpectraMax 190 microplate spectrophotometer (Molecular Devices Corp, CA, USA).

Techniques: Concentration Assay

Journal: Pediatric diabetes

Article Title: Relationship of Adiponectin and Leptin with Autoimmunity in Children with New Onset Type 1 Diabetes (T1D): A Pilot Study

doi: 10.1111/pedi.12267

Figure Lengend Snippet: Longitudinal Adiponectin Levels

Article Snippet: Adiponectin and Leptin assays Serum total adiponectin levels were determined by radioimmunoassay (Human adiponectin RIA kit, Linco Research, St Charles, Missouri, USA).

Techniques:

Journal: Pediatric diabetes

Article Title: Relationship of Adiponectin and Leptin with Autoimmunity in Children with New Onset Type 1 Diabetes (T1D): A Pilot Study

doi: 10.1111/pedi.12267

Figure Lengend Snippet: Racial differences in longitudinal adipocytokine values

Article Snippet: Adiponectin and Leptin assays Serum total adiponectin levels were determined by radioimmunoassay (Human adiponectin RIA kit, Linco Research, St Charles, Missouri, USA).

Techniques:

Journal: EMBO Molecular Medicine

Article Title: Photoreceptor glucose metabolism determines normal retinal vascular growth

doi: 10.15252/emmm.201707966

Figure Lengend Snippet: The mouse neonates received intraperitoneal injection of STZ and schematic graph shows mouse retinal vessel development after birth (left). GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Representative images of the deep vascular network in isolectin‐stained (red, top) retinal whole mounts of HAR and controls. Scale bar, 50 μm (top) and 1 mm (bottom). The total retinal area is outlined in yellow and deep vascular coverage is highlighted in red (bottom, grayscale). Quantification of deep vascular network of HAR and controls. n = 17–27 retinas/group. Data presented as mean ± SEM, unpaired t ‐test. Blood glucose ( n = 19–27 retinas/group), serum insulin (ELISA) ( n = 4–7 pooled samples/group) and triglyceride (TG) concentrations ( n = 7/group) of HAR and controls. Data presented as mean ± SEM, unpaired t ‐test. Data information: See also Fig .

Article Snippet: All serum samples were diluted 1:306 and APN levels assayed using a human adiponectin ELISA kit (E091M, Mediagnost, Reutlingen, Germany).

Techniques: Injection, Staining, Enzyme-linked Immunosorbent Assay

Journal: EMBO Molecular Medicine

Article Title: Photoreceptor glucose metabolism determines normal retinal vascular growth

doi: 10.15252/emmm.201707966

Figure Lengend Snippet: A Left: serum APN levels (ELISA) ( n = 8–9 retinas/group); right: retinal apn (qRT–PCR) ( n = 6 retinas/group) of HAR and controls. B Left: retinal cross‐sectional layers for laser capture microdissection (LCM: DAPI for nuclei, blue; isolectin for vessels, red); center: mRNA levels of AdipoR1 and AdipoR2 in retinal neuronal layers and vessels. n = 3 pooled retinas/group. Right: AdipoR1 mRNA in HAR and control retinas. n = 5 retinas/group. C Control and HAR eyes in WT and Apn −/− mice. Left: representative images of DAPI‐stained hyaloid vessels (blue). Right: quantification of preserved hyaloid vessels branching from the hyaloid artery (white arrow). Scale bar, 1 mm. n = 8–30 retinas/group. D Left: Fundus photograph focused to show persistent hyaloid vessels (white arrows) (green, fluorescein AK‐FLUOR) in WT and Apn −/− mice at P30 ( n = 37–48 retinas/group); right: pie graph of percentage of eyes examined with persisting hyaloid. E Left: In WT and Apn −/− representative images of deep retinal vasculature (lectin, red) in whole‐mounted retinas; right: quantification of deep vessels ( n = 14–22 retinas/group). F, G Left: Representative images of deep retinal vasculature (lectin, red) in whole‐mounted retinas of WT hyperglycemic (HAR) mice with recombinant mouse APN treatment (F) ( n = 6–10 retinas/group) or with AdipoRon treatment (G). Right: quantification of deep retinal vasculature ( n = 7–14 retinas/group). Data information: Scale bars, 50 μm (B, E–G) or 1 mm (C). Data presented as mean ± SEM; unpaired t ‐test (A, B, E–G) or ANOVA (C). See also Fig .

Article Snippet: All serum samples were diluted 1:306 and APN levels assayed using a human adiponectin ELISA kit (E091M, Mediagnost, Reutlingen, Germany).

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Laser Capture Microdissection, Control, Staining, Recombinant

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Utility of hemoglobin A1c in detecting risk of type 2 diabetes: comparison of hemoglobin A1c with other biomarkers

doi: 10.3164/jcbn.19-16

Figure Lengend Snippet: Correlation between the glucose and inslurin resistance during fasting and OGTT and fasting levels of multi-biomaekers

Article Snippet: HbA1c, glucose, insulin, leptin, adiponectin, RBP4, glycoalbumin, and hs-CRP were measured using commercially available ELISA kits {HbA1c: RAPIDIA Auto HbA1c-L [Fujirebio Inc. (Tokyo, Japan)]; glucose: Cica liquid GLU J [KANTO CHEMICAL Co., Inc. (Tokyo, Japan)]; insulin: Lumipulse Presto Insulin (Fujirebio Inc.); leptin: Human leptin RIA kit [Millipore Co., Inc. (Tokyo, Japan)]; adiponectin: CircuLexTM Human adiponectin ELISA Kit CY-8050 [MBL Co., Ltd. (Nagano, Japan)]; RBP4: CircuLex Human RBP4 ELISA Kit (MBL Co., Ltd.); glycoalbumin: Lucia GA-L [Asahi KASEI Pharma Co., Inc. (Tokyo, Japan)]; and hs-CRP: CircuLexTM Human HS-CRP ELISA Kit CY-8071 (MBL Co., Ltd.)}.

Techniques:

Journal: Cell Metabolism

Article Title: Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets

doi: 10.1016/j.cmet.2018.02.007

Figure Lengend Snippet: Activation of the Wnt/β-Catenin and PPARγ Pathway in cDC1 and cDC2 DCs, Respectively, Suppresses TLR4-Induced Inflammation cDC1 and cDC2 were purified from spleen and VAT by cell sorting as CD11c hi MHCII + GFP + cells. (A and B) Cells were incubated overnight with 5 μg GLA in the presence of β-catenin (SB) or PPARγ (RGZ) agonist or DMSO control. IL-6 (A) and IL-10 (B) production in supernatants was measured by ELISA. Bars represent the mean ± SEM and are representative of three independent experiments (n = 9). (C) A total of 300 μg VAT was isolated from lean mice and cultured overnight (Sup) or immediately lysed (Lys), and Wnt10b production was analyzed by ELISA. Bars represent the mean ± SEM (n = 3). (D) Total GFP + cDCs were purified and cultured overnight with 100 ng/mL recombinant Wnt10b or SB agonist as positive control; IL-10 was determined in the supernatant by ELISA (n = 9). (E and F) As described in (D), but in addition, cells were stimulated with GLA in the presence of recombinant Wnt10b; the production of IL-6 (E) and IL-10 (F) was determined in the supernatant by ELISA. Bars represent the mean ± SEM and are representative of three independent experiments (n = 9). Statistical analysis was performed with Student’s test, ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.0005.

Article Snippet: Mouse Adiponectin ELISA kit , Merck-Millipore , RRID: AB_2651034.

Techniques: Activation Assay, Purification, FACS, Incubation, Control, Enzyme-linked Immunosorbent Assay, Isolation, Cell Culture, Recombinant, Positive Control

Journal: Cell Metabolism

Article Title: Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets

doi: 10.1016/j.cmet.2018.02.007

Figure Lengend Snippet: Ctnnb1 −/− and Pparγ −/− Mice Showed No Statistical Significance in Metabolic Parameters on Chow Diet Ctnnb1 −/− and Pparγ −/− mice were fed a chow diet. (A) Graph shows representative body weights from three independent experiments. (B) VAT content was calculated as percentage of body weight. Graphs represent the mean ± SEM and are representative of three independent experiments. (C and D) Fasting insulin (C) and adiponectin (D) were detected by ELISA. Bars represent the mean ± SEM and are representative of three independent experiments. Statistical analysis was performed with Student’s test. (E and F) Whole-body glucose homeostasis measured in (E) Ctnnb1 −/− , (F) Pparγ −/− , and WT mice on chow diet by i.p. glucose tolerance tests (GTTs; n = 10) and insulin tolerance tests (ITTs; n = 10). Statistical significance at different time points analyzed by two-way ANOVA with Bonferroni’s post-test, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001.

Article Snippet: Mouse Adiponectin ELISA kit , Merck-Millipore , RRID: AB_2651034.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: Cell Metabolism

Article Title: Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets

doi: 10.1016/j.cmet.2018.02.007

Figure Lengend Snippet: Deletion of β-Catenin and Pparγ on cDCs Alters Glucose Homeostasis (A) Body weight of Ctnnb1 −/− , Pparγ −/− , and WT mice fed WD for 12 weeks. (B–D) Serum leptin (B), adiponectin (C), and insulin (D) values from fasted mice representative of two independent experiments (n = 10). Statistical analysis was performed with Student’s test. (E–H) Systemic glucose homeostasis was measured by ITT (E and G) in Ctnnb1 −/− (E), Pparγ −/− (G), and WT control mice fed a WD. Similarly, GTT (F and H) was assessed in Ctnnb1 −/− (F) and Pparγ −/− (H) compared to control mice. Statistical significance at different time points was analyzed by two-way ANOVA with Bonferroni’s post-test (n = 10). (I and J) VAT Akt activation followed acute insulin injection. Western blot analysis of VAT extracts showing phosphor-(Ser473) Akt (p-Akt) levels under control and insulin treatment in Ctnnb1 −/− (I) and Pparγ −/− (J) compared to WT mice. Graphs show densitometry of p-Akt/Akt ratios. ∗ p < 0.05, ∗∗ p < 0.01, and ∗∗∗ p < 0.0005. See also Figure S5 .

Article Snippet: Mouse Adiponectin ELISA kit , Merck-Millipore , RRID: AB_2651034.

Techniques: Control, Activation Assay, Injection, Western Blot

Journal: Cell Metabolism

Article Title: Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets

doi: 10.1016/j.cmet.2018.02.007

Figure Lengend Snippet:

Article Snippet: Mouse Adiponectin ELISA kit , Merck-Millipore , RRID: AB_2651034.

Techniques: Recombinant, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Staining, Plasmid Preparation, Western Blot, RNA Sequencing, Gene Expression, Software

Journal: PLoS ONE

Article Title: Gender difference in NASH susceptibility: Roles of hepatocyte Ikkβ and Sult1e1

doi: 10.1371/journal.pone.0181052

Figure Lengend Snippet: Wild type (Wt), myeloid IKKβ deficient ( IkbkbΔmye ), or hepatocyte IKKβ deficient ( IkbkbΔhep ) mice in both genders, were fed regular chow (Control) or high cholesterol and saturated fat diet (HCFD) from 2.5 months of age for 20 weeks. (A) Immunoblotting of whole liver lysate and (B) densitometric analysis of pAMPK/AMPK, nSREBP-1c, PPARδ, pJNK-1/JNK-1, and pJNK-2/JNK-2. (C) Plasma adiponectin levels measured by ELISA. (D) Real-time PCR results of PPAR α, δ, and γ genes. *p<0.05, **p<0.01, ***p<0.001 compared to Control diet within gender and within genotype. †p<0.05, ††p<0.01 compared to other genotype within gender and within diet.

Article Snippet: Plasma ALT, insulin and adiponectin concentrations were measured by ELISA kits (ALT: Sigma Diagnostic, St. Louis, MO, insulin: Shibayagi Co., Gunma, Japan, adiponectin: ALPCO Diagnostics, Salem, NH, respectively).

Techniques: Control, Western Blot, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction